Revolutionizing metabarcoding for practitioners and conservationists

Metabarcoding has revolutionized modern biodiversity research from environmental DNA to bulk barcoding and microbiome research. But to take part in this revolution, one needs vast molecular experience and access to vast laboratory resources, leaving conservationists, practitioners and local authorities behind.

In our recent publication An isothermal workflow for low-cost and PCR-free field-based community metabarcoding published in Methods in Ecology and Evolution, Samplify scientists have cracked the code to faster, simpler and cheaper metabarcoding. The open access publication introduces our novel open-source environmental DNA filtration device (now also available for sale on our Equipment page) and presents a new DNA extraction method that takes less than one minute in the field. But most importantly, we discovered how the fast, isothermal Recombinase Polymerase Amplification can be used to replace PCR in the field. Combined with Nanopore sequencing, we have now provided a complete toolkit to use environmental DNA metabarcoding with miniaturized equipment directly on site.

But how does it work? We provide hands-on protocols and DIY descriptions for equipment and workflows in the publication (don´t overlook the Supplement). Are ready-made solutions more suitable for your company or institute? Contact a Samplifylab scientist today to learn more about available field-lab equipment, training options and custom research as well as the already available metabarcoding services.